Variations in the content of satellite III (1q12) and ribosomal repeat in the DNA of blood leukocytes of children with autism spectrum disorders during therapy

Variations in the content of satellite III (1q12) and ribosomal repeat in the DNA of blood leukocytes of children with autism spectrum disorders during therapy

 

Authors

 

E.S. Ershova

Federal State Budgetary Scientific Institution “Medical and Genetic Research Center named after Academician N.P. Bochkov”, Moscow, Russian Federation

Yu.M. Chudakova

Federal State Budgetary Scientific Institution “Medical and Genetic Research Center named after Academician N.P. Bochkov”, Moscow, Russian Federation

N.N. Veiko

Federal State Budgetary Scientific Institution “Medical and Genetic Research Center named after Academician N.P. Bochkov”, Moscow, Russian Federation

A.V. Martynov

Federal State Budgetary Scientific Institution “Medical and Genetic Research Center named after Academician N.P. Bochkov”, Moscow, Russian Federation

S.E. Kostyuk

Federal State Budgetary Scientific Institution “Medical and Genetic Research Center named after Academician N.P. Bochkov”, Moscow, Russian Federation

S.V. Kostyuk

Federal State Budgetary Scientific Institution “Medical and Genetic Research Center named after Academician N.P. Bochkov”, Moscow, Russian Federation

E.E. Balakireva

Federal State Budgetary Scientific Institution “Mental Health Research Center”, Russian Academy of Sciences, Moscow, Russian Federation

O.V. Shushpanova

Federal State Budgetary Scientific Institution “Mental Health Research Center”, Russian Academy of Sciences, Moscow, Russian Federation

S.G. Nikitina

Federal State Budgetary Scientific Institution “Mental Health Research Center”, Russian Academy of Sciences, Moscow, Russian Federation

T.E. Blinova

Federal State Budgetary Scientific Institution “Mental Health Research Center”, Russian Academy of Sciences, Moscow, Russian Federation

N.S. Shalina

Federal State Budgetary Scientific Institution “Mental Health Research Center”, Russian Academy of Sciences, Moscow, Russian Federation

A.A. Koval-Zaitsev

Federal State Budgetary Scientific Institution “Mental Health Research Center”, Russian Academy of Sciences, Moscow, Russian Federation

M.V. Ivanov

Federal State Budgetary Scientific Institution “Mental Health Research Center”, Russian Academy of Sciences, Moscow, Russian Federation

 

https://doi.org/10.26617/1810-3111-2024-4(125)-17-26

 

Journal: Siberian Herald of Psychiatry and Addiction Psychiatry. 2024; 4 (125):  17-26.

 

Abstract

Background. It is known that exacerbation of chronic mental diseases is accompanied by a high level of psy-choemotional stress, and is also associated with activation of nonspecific inflammation and increased oxidative stress. Children with ASD are more susceptible to the adverse effects of stress than average children due to the high level of comorbidity of ASD with other disorders. Earlier studies described the relationship between oxidative stress and repetitive DNA sequences (repeats) that make up a major part of the human genome. The content of satellite III (1q12) in the genomes of children with ASD and the effect of therapy on this parameter have not been determined. In this study, the amount of ribosomal repeat and satellite III (1q12) in the DNA of blood leukocytes of children with ASD who sought help at the Department of Child Psychiatry due to worsening their mental state (increasing concomitant behavioral and/or mood disorders) was established for the first time. The effect of the therapy on the content of these repeats was analyzed. Objective: to analyze the content of genomic repeats (ribosomal, satellite III ‒ 1q12) in DNA samples of blood leukocytes of children with ASD and concomitant disorders in the acute stage in order to analyze the effect of the therapy on the content of these repeats. Patients and Methods. The main study group included 35 children aged 4 to 12 years (mean age 7.2±2.2 years) with the main diagnosis according to ICD-10: childhood autism (F84.0), atypical autism (F84.1) and with a concomitant diagnosis of other anxiety disorders (F41), reaction to severe stress, and adjustment disorders (F43), unspecified mental retardation with significant impairment of behaviour requiring attention or treatment (F7x.1), mixed disorders of conduct and emotions (F92). The control group consisted of 91 children and 100 adults. Patients were selected using the clinical and psychopathological method. Venous blood samples were taken: sample 1 ‒ DNA samples of children before the start of new therapy, samples 2, 3 and 4 ‒ DNA samples obtained by week 2, 4 and 6 of the therapy. DNA was isolated by extraction with organic solvents. The content of three repeats in DNA was determined by non-radioactive quantitative hybridization. The data were analyzed using the StatPlus2007 Professional software, MedCalc, Excel Microsoft Office, StatGraph. Results. DNA samples of children with ASD did not differ in the content of ribosomal repeats from the control group of children of the same age. The content of satellite III varied significantly in most patients during therapy: it increased by 1.1-2 times in 18 patients (51%), decreased by 1.1-4.3 times in 12 patients (34%), and did not change in 5 patients. The content of the satellite increased in case of an initially low repeat level and decreased in case of an initially high level. In sample 4, the distribution for S values was virtually identical to the control distribution. Conclusion. Irrespective from the initial values, under antipsychotic therapy, the content of satellite III (1q12) in the blood of children with ASD and exacerbation of concomitant symptoms stabilize at the level of the control group. These changes are likely associated with an improvement in mental state and a decrease in the overall stress level. The R parameter (ribosomal repeat) did not change throughout this study.

 

Keywords: autism spectrum disorders, children, content variations, satellite III (1q12), ribosomal repeat, blood leukocyte DNA, psychopharmacotherapy.

 

Article (pdf)

 

Contacts

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Materials  

For citation: Ershova E.S., Chudakova Yu.M., Veiko N.N., Martynov A.V., Kostyuk S.E., Kostyuk S.V., Balakireva E.E., Shushpanova O.V., Nikitina S.G., Blinova T.E., Shalina N.S., Koval-Zaitsev A.A., Ivanov M.V. Variations in the content of satellite III (1q12) and ribosomal repeat in the DNA of blood leukocytes of children with autism spectrum disorders during therapy. Siberian Herald of Psychiatry and Addiction Psychiatry.2024; 4 (125): 17-26. https://doi.org/10.26617/1810-3111-2024-4(125)-17-26

 

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