Red blood cell indices in patients with alcoholism with different levels of peripheral markers of oxidative stress

Red blood cell indices in patients with alcoholism with different levels of peripheral markers of oxidative stress

 

Authors

 

V.D. Prokopieva

Mental Health Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russian Federation

T.P. Vetlugina

Mental Health Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russian Federation

A.I. Mandel

Mental Health Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russian Federation

E.V. Epimakhova

Mental Health Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russian Federation

L.I. Martynenko

Mental Health Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russian Federation

S.A. Vorobieva

Mental Health Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russian Federation

 

https://doi.org/10.26617/1810-3111-2024-3(124)-5-11

 

Journal: Siberian Herald of Psychiatry and Addiction Psychiatry. 2024; 3 (124):  5-11.

 

Abstract

Introduction. High levels of peripheral markers of oxidative stress (OS) in alcoholism are not detected in all patients. The study of biological parameters in groups of patients with alcoholism, differing in the level of OS severity, is necessary for a better understanding of the role of OS in the development of the disease, as well as for deciding on the advisability of prescribing antioxidant therapy. Objective. To conduct a comparative analysis of erythrocyte parameters in patients with alcoholism with different levels of peripheral markers of oxidative stress. Patients and Methods. The study was conducted at the Addictive States Department of Mental Health Research Institute of Tomsk National Research Medical Center. The study sample included 57 male patients with alcoholism, mean age 44.9±9.0 years, with a diagnosis according to ICD-10: Mental and behavioral disorders due to the use of alcohol; withdrawal state (F10.302). Laboratory studies were performed by days 3-5 of hospital admission after alcohol detoxification. Based on the concentration of oxidized protein products (carbonylated proteins, CP) and lipid peroxidation products (TBA-reactive products, TBA-RP) in blood plasma, the patients in the general sample were divided into two groups: 1) OS group (n=42) ‒ CP and/or TBA-RP levels in blood plasma exceeded control levels by 20% or more, 2) non-OS group (n=15) ‒ CP and TBA-RP levels in blood plasma did not exceed control levels by more than 20%. CP was determined in a reaction with 2,4-dinitrophenylhydrazine, TBA-RP – by the reaction of lipid peroxidation products with thiobarbituric acid. Hematological blood analysis was performed on a Micros 60 hematological analyzer. Results. A statistically significant (p=0.045) increase in the volume of erythrocytes was found in patients of OS group compared to patients of non-OS group. Conclusion. The findings showed that macrocytosis in alcoholism may be associated with oxidative stress. The final data can be used in narcological practice when prescribing adjuvant antioxidant therapy based on a general clinical blood test without additional, more labor-intensive and expensive determination of the oxidative status.

 

Keywords: oxidative stress, peripheral markers, erythrocytes, alcoholism.

 

Article (pdf)

 

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Materials 

For citation: Prokopieva V.D., Vetlugina T.P., Mandel A.I., Epimakhova E.V., Martynenko L.I., Vorobieva S.A. Red blood cell indices in patients with alcoholism with different levels of peripheral markers of oxidative stress. Siberian Herald of Psychiatry and Addiction Psychiatry.2024; 3 (124): 5-11. https://doi.org/10.26617/1810-3111-2024-3(124)-5-11

 

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