Time-resolved tryptophan fluorescence as an approach to detect conformational abnormalities of the serum albumin molecule in melancholic depression

Time-resolved tryptophan fluorescence as an approach to detect conformational abnormalities of the serum albumin molecule in melancholic depression

 

Authors

 

M.G. Uzbekov

Moscow Research Institute of Psychiatry ‒ Branch of FSBI “V.P. Serbsky National Medical Research Center of Psychiatry and Narcology” of the Ministry of Health of the Russian Federation, Moscow, Russian Federation

T.I. Syreyshchikova

Р.N. Lebedev Physical Institute of the Russian Academy of Sciences, Moscow, Russian Federation

N.V. Smolina

Moscow Research Institute of Psychiatry ‒ Branch of FSBI “V.P. Serbsky National Medical Research Center of Psychiatry and Narcology” of the Ministry of Health of the Russian Federation, Moscow, Russian Federation

S.N. Shikhov

Moscow Research Institute of Psychiatry ‒ Branch of FSBI “V.P. Serbsky National Medical Research Center of Psychiatry and Narcology” of the Ministry of Health of the Russian Federation, Moscow, Russian Federation

V.V. Brilliantova

Moscow Research Institute of Psychiatry ‒ Branch of FSBI “V.P. Serbsky National Medical Research Center of Psychiatry and Narcology” of the Ministry of Health of the Russian Federation, Moscow, Russian Federation

V.V. Kryukov

Moscow Research Institute of Psychiatry ‒ Branch of FSBI “V.P. Serbsky National Medical Research Center of Psychiatry and Narcology” of the Ministry of Health of the Russian Federation, Moscow, Russian Federation

G.E. Dobretsov

Moscow Research Institute of Psychiatry ‒ Branch of FSBI “V.P. Serbsky National Medical Research Center of Psychiatry and Narcology” of the Ministry of Health of the Russian Federation, Moscow, Russian Federation

 

https://doi.org/10.26617/1810-3111-2023-3(120)-5-11

 

Journal: Siberian Herald of Psychiatry and Addiction Psychiatry. 2023; 3 (120):  5-11.

 

Abstract

Introduction. Approaches to therapy, predicting the dynamics and evaluating the effectiveness of psychopharmacotherapy of mood disorders is an extremely important problem in biological psychiatry. The fluorescence of a tryptophan residue is very sensitive to changes in the structure of its environment in the protein. In human serum albumin (HSA), radiation at wavelengths from 295 to 305 nm absorbs mainly tryptophan (Trp214), which, as an internal probe of albumin molecules, makes it possible to selectively observe the state of the albumin molecule and its possible conformational changes. Objective: to study the kinetics of fluorescence decay of HSA tryptophan in patients with melancholic depression using subnanosecond fluorescence spectroscopy. Material. We examined 14 patients (the main group) diagnosed with ICD-10 melancholic depression (MD), their psychopathological state was assessed as a depressive episode within the framework of bipolar affective disorder (F31.3) or recurrent depressive disorder (F33.1). The control group consisted of 14 volunteers without morbid changes according to the results of express diagnostics of clinical-psychopathological and clinical-biochemical studies. There were no statistically significant differences between the examined groups in terms of age and sex parameters. Methods. Psychometric quantification of the severity of depressive symptoms was assessed using the Hamilton Depression Rating Scale (HAMD-21, HDRS-21) and Anxiety Rating Scale (HARS). The examination was carried out on the first day of the patient's admission to the hospital before the start of active psychopharmacological treatment. Tryptophan fluorescence decay kinetics were measured on a Laser Emission Diode setup with a pulsed light source. The excitation wavelength was 290±10 nm. Results. Analysis of all parameters of tryptophan fluorescence decay in blood serum samples of the examined groups before the start of therapy showed that the average values of the A1 and A3 amplitudes in the serum of patients with MD of the main group were statistically significantly (p=0.01) lower than in the control group of volunteers (for A1 ‒ 378 versus 440, for A3 ‒ 285 versus 327, respectively). Conclusion. Based on the results of the study, it was demonstrated that conformational changes in the albumin molecule in patients with mental pathology (melancholic depression) can be detected using subnanosecond fluorescence spectroscopy of fluorescent tryptophan residua, which confirms the scientific novelty, practical significance of the work and the need to continue this direction.

 

Keywords: tryptophan, subnanosecond fluorescence spectroscopy, laser, fluorescence decay, human serum albumin, conformation, melancholic depression.

 

Article (pdf)

 

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Materials  

 

For citation: Uzbekov M.G., Syreyshchikova T.I., Smolina N.V., Shikhov S.N., Brilliantova V.V., Kryukov V.V., Dobretsov G.E. Time-resolved tryptophan fluorescence as an approach to detect conformational abnormalities of the serum albumin molecule in melancholic depression.Siberian Herald of Psychiatry and Addiction Psychiatry.2023; 3 (120): 5-11. https://doi.org/10.26617/1810-3111-2023-3(120)-5-11.

 

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