Influence of immunocompetent cells modulated ex vivo by chlorpromazine on hematopoiesis during aggression

Influence of immunocompetent cells modulated ex vivo by chlorpromazine on hematopoiesis during aggression

 

Authors

 

E.V. Markova

Federal State Budgetary Scientific Institution "Scientific Research Institute of the Fundamental and Clinical Immunology", Novosibirsk, Russian Federation

E.V. Serenko

Federal State Budgetary Scientific Institution "Scientific Research Institute of the Fundamental and Clinical Immunology", Novosibirsk, Russian Federation

L.B. Toporkova

Federal State Budgetary Scientific Institution "Scientific Research Institute of the Fundamental and Clinical Immunology", Novosibirsk, Russian Federation

M.A. Knyazheva

Federal State Budgetary Scientific Institution "Scientific Research Institute of the Fundamental and Clinical Immunology", Novosibirsk, Russian Federation

I.V. Savkin

Federal State Budgetary Scientific Institution "Scientific Research Institute of the Fundamental and Clinical Immunology", Novosibirsk, Russian Federation

E.V. Goiman

Federal State Budgetary Scientific Institution "Scientific Research Institute of the Fundamental and Clinical Immunology", Novosibirsk, Russian Federation

I.A. Orlovskaya

Federal State Budgetary Scientific Institution "Scientific Research Institute of the Fundamental and Clinical Immunology", Novosibirsk, Russian Federation

 

https://doi.org/10.26617/1810-3111-2023-3(120)-12-20

 

Journal: Siberian Herald of Psychiatry and Addiction Psychiatry. 2023; 3 (120):  12-20.

 

Abstract

Background. Prolonged stress, including chronic social stress, results in anxiety and aggressive or depression-like behavior. Neuroendocrine signals formed during prolonged social stress cause the activation of myelopoiesis, which is accompanied by increased migration of monocytes from the bone marrow to the periphery and the acquisition of an “inflammatory” M1-phenotype by them. Some of these monocytes also migrate to the brain, activating endothelial cells and microglia, which leads to the formation of a neuroinflammatory status and behavioral changes. Previously, we obtained priority data on the possibility of editing patterns of aggressive behavior by syngeneic immunocompetent cells modulated ex vivo with chlorpromazine and showed the cytokine-mediated mechanisms of this effect. Objective: assessment of bone marrow hematopoiesis and cellular composition of peripheral blood in experimental animals during the formation of aggressive behavior due to chronic social conflict and after its editing by syngeneic immunocompetent spleen cells modulated ex vivo with chlorpromazine. Materials and Methods. Male (CBAxC57Bl/6)F1 mice with aggressive behavior, formed as a result of 20-fold experience of victories in daily agonistic interactions with a passive partner, were transplanted with syngeneic spleen immunocompetent cells with functional activity modulated by chlorpromazine ex vivo. In aggressive mice and aggressive recipients, the number of bone marrow hematopoietic progenitors was assessed according to the recommendations of Stem Cell Technologies (Canada) as well as the cellular composition of peripheral blood.Results. In accordance with the data obtained from the studies, a significant increase in the bone marrow of the granulocytic-macrophage (CFU-GM) direction of hematopoietic stem cell differentiation in aggressive males (CBA×C57Bl/6)F1 was shown, which was accompanied by an increase in the peripheral blood of mice in populations of leukocytes, monocytes, segmented neutrophils and lymphocytes. Splenocytes isolated from aggressive donors (CBA×C57Bl/6)F1 and modulated ex vivo with chlorpromazine, after transplantation into syngeneic aggressive recipients, had a corrective effect on these parameters, reducing them almost to the level characteristic of intact animals of the corresponding age. The mechanisms of the hematopoiesis-correcting effect of immunocompetent spleen cells modulated by chlorpromazine in an aggressive state are discussed.

 

Keywords: experimental animals, aggressive behavior, immunocompetent cells, chlorpromazine, hematopoiesis, bone marrow, peripheral blood.

 

Article (pdf)

 

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Materials  

For citation: Markova E.V., Serenko E.V., Toporkova L.B., Knyazheva M.A., Savkin I.V., Goiman E.V., Orlovskaya I.A. Influence of immunocompetent cells modulated ex vivo by chlorpromazine on hematopoiesis during aggression.Siberian Herald of Psychiatry and Addiction Psychiatry.2023; 3 (120): 12-20. https://doi.org/10.26617/1810-3111-2023-3(120)-12-20

 

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